Isolation and Biosynthesis of an Azoxyalkene Compound Produced by a Multiple Gene Disruptant of Streptomyces rochei
CHEMBIOCHEM Volume 16 Issue 15
Page 2237-2243
published_at 2015-10-12
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| File | |
| Title ( eng ) |
Isolation and Biosynthesis of an Azoxyalkene Compound Produced by a Multiple Gene Disruptant of Streptomyces rochei
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| Creator |
Kunitake Hirofumi
Hiramatsu Takahiro
Kinashi Haruyasu
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| Source Title |
CHEMBIOCHEM
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| Volume | 16 |
| Issue | 15 |
| Start Page | 2237 |
| End Page | 2243 |
| Abstract |
Streptomyces rochei 7434AN4 predominantly produces lankacidin and lankamycin under normal culture conditions, suggesting that other biosynthetic gene clusters for secondary metabolites are silent. To exploit silent metabolites of strain 7434AN4, we constructed mutant KA57, a multiple disruptant of the transcriptional repressor gene srrB together with the biosynthesis genes for both antibiotics. Mutant KA57 accumulated a compound (KA57-A) with a strong UV absorption at 235 nm, which was not detected in the parent strain or other mutants. Various spectroscopic analyses revealed that KA57-A is an azoxyalkene compound of a molecular formula of C10H20N2O3 with the R configuration at C-2. Biosynthesis of KA57-A was also studied by feeding with labeled acetates, amino acids, and 1-hexylamine. The hexenyl moiety (C1’-C6’) was derived from fatty acid, while the 3-amino-butan-1,2-diol moiety (C1-C4) was derived from C-2 of acetate (C1) and serine (C2-C4). Incorporation of [1,1-2H2]1-hexylamine indicated that C1’-C2’ dehydrogenation occurs at the final step of biosynthesis.
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| Keywords |
biosynthesis
genetic engineering
azoxyalkene
natural products
Streptomyces
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| Descriptions |
アクセプト後にアブストラクト等変更あり。
This work was supported by a Grants‐in‐Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and a Noda Institute for Scientific Research Grant.
Supporting information for this articleis available on the WWW under http://dx.doi.org/10.1002/cbic.201500393.
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Wiley‐VCH Verlag
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| Date of Issued | 2015-10-12 |
| Rights |
© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
This is the peer reviewed version of the following article: CHEMBIOCHEM. 2015 Oct 12;16(15):2237-2243, which has been published in final form at https://doi.org/10.1002/cbic.201500393. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 1439-4227
[ISSN] 1439-7633
[DOI] 10.1002/cbic.201500393
[PMID] 26300120
[DOI] https://doi.org/10.1002/cbic.201500393
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