Structural and functional insights into thermally stable cytochrome c' from a thermophile
Protein Science 26 巻 4 号
737-748 頁
2017-03-06 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Structural and functional insights into thermally stable cytochrome c' from a thermophile
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| 作成者 |
Oki Hiroya
Kawahara Kazuki
Yamane Daisuke
Yamanaka Masaru
Maruno Takahiro
Kobayashi Yuji
Masanari Misa
Wakai Satoshi
Nishihara Hirofumi
Ohkubo Tadayasu
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| 収録物名 |
Protein Science
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| 巻 | 26 |
| 号 | 4 |
| 開始ページ | 737 |
| 終了ページ | 748 |
| 抄録 |
Thermophilic Hydrogenophilus thermoluteolus cytochrome c0 (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c0 (AVCP), which has a homo-dimeric structure and ligand-binding ability. To understand the thermal stability mechanism and ligand-binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo-dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 A ° . In the PHCP structure, six specific residues appeared to strengthen the heme-related and subunit–subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit–subunit interactions were more severely destabilized than ones having altered heme-related interactions. The PHCP structure further revealed a ligand-binding channel and a penta-coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme-related and subunit–subunit interactions with conservation of the ligand-binding ability.
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| 著者キーワード |
protein stability
cytochrome c
thermophile
protein structure
mutagenesis
ligand-binding
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| 内容記述 |
This work was performed under the Cooperative Research Program of the “Network Joint Research Center for Materials and Devices”.
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Wiley
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| 発行日 | 2017-03-06 |
| 権利情報 |
© 2017 The Authors Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0961-8368
[ISSN] 1469-896X
[DOI] 10.1002/pro.3120
[PMID] 28097774
[DOI] https://doi.org/10.1002/pro.3120
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