Novel Assay System Favorable for the Study of Cell-to-Cell Transmission of HIV-1 and Its Application to the Evaluation of Anti-HIV Drugs

Inoue Yoshio

Kanamori Tatsuyuki

Fujimoto Yasuhiro

Sugiyama Masanori

Yoshida Tetsuya

Biological and Pharmaceutical Bulletin

The cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) was studied using MOLT-4 cells chronically infected with a variant strain of HIV-1SF-2 (MOLT-4/HIV-1SF-2H) and CD4+ human lymphoid MT-4 cells. MOLT-4/HIV-1SF-2H cells produced less than 1 TCID50 infectious particles per 105 cells per day as determined by the cytopathogenicity in MT-4 cells. However, the expression of envelope glycoproteins gp120 and gp41 on the MOLT-4/HIV-1SF-2H cell membrane was satisfactory for syncytium formation with the uninfected MOLT-4 cells. When MOLT-4/HIV-1SF-2H and MT-4 cells were co-cultured, severe cytopathogenicity was observed in MT-4 cells without being accompanied by the formation of multi-nucleated cells. Thus, the system consisting of MOLT-4/HIV-1SF-2H and MT-4 cells is convenient for exclusive study of the mechanism of cell-to-cell transmission of HIV-1. Using various compounds, it was confirmed that cell-to-cell transmission required both gp120/gp41-CD4 binding and de novo DNA synthesis.

human immunodeficiency virus

cell-to-cell transmission

syncytium formation

de novo DNA synthesis

Medical sciences [ 490 ]

eng

The Pharmaceutical Society of Japan

© 1995 Pharmaceutical Society of Japan

[ISSN] 0918-6158

[ISSN] 1347-5215

[NCID] AA10885497

[DOI] 10.1248/bpb.18.920

[DOI] https://doi.org/10.1248/bpb.18.920