Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains
Journal of Microbiological Methods Volume 89 Issue 1
Page 53-56
published_at 2012-04
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| File | |
| Title ( eng ) |
Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains
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| Creator | |
| Source Title |
Journal of Microbiological Methods
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| Volume | 89 |
| Issue | 1 |
| Start Page | 53 |
| End Page | 56 |
| Abstract |
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.
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| Keywords |
Agrobacterium
plasmid removal
Ti plasmid
Toxin-antitoxin system
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| Descriptions |
This work was supported in part by the Japanese Ministry of Education, Science, Sports, and Culture, Grant-in-Aid for Scientific Research (grant 20570221).
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| NDC |
Zoology [ 480 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Elsevier B.V.
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| Date of Issued | 2012-04 |
| Rights |
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0167-7012
[ISSN] 1872-8359
[DOI] 10.1016/j.mimet.2012.01.019
[DOI] https://doi.org/10.1016/j.mimet.2012.01.019
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