A Diluent for Deep Freezing Preservation of Fowl Spermatozoa

鶏精子の凍結保存用希釈液について

Watanabe Moriyuki

Terada Takato

Shirakawa Yoshihisa

広島大学水畜産学部紀要

Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University

著者らはこれまでの実験で,グリセリンの最終濃度が7%になるよう調整した卵黄ブドウ糖希釈液を用いて鶏精液を4倍に希釈して凍結保存を実施してきたが,その受精率は50～60％の水準にとどまった。今回この従来の希釈液を対照区とし,新たにnon‐yolk5.7%ブドウ糖液を試験区としてそれぞれ4倍希釈で凍結保存を実施し,融解後の精子活力,奇形率および受精率について比較検討した結果,融解後の精子活力については両区間に明らかな有意差が認められた(P>0.01)が奇形率においては有意差は認あられなかった(P<0.05)。試験区の1週目受精率は平均74.0%,2週目受精率は平均55.9%で対照区の1週目平均受精率52.9%,2週目平均受精率32.8％に比らべかなり良好な結果を示した。

以上の結果,鶏精子の凍結保存においてもnon‐yolkブドウ糖液は好結果を与えるもののようである。

The motility, deformity and fertility of the frozen semen which were diluted 1 to 3 with a non-yolk 5.7% glucose solution and the previous diluent containing 7% glycerol in its final concentration were compared mutually. The results are as follows:

The motility and the deformity percent of thawed semen treated by a non-yolk glucose solution was 85.6 ± 8.0 percent and 8.4 ± 2.0 percent on the average. The Motility was higher and the deformed sperm average lower than those in the previous diluent. The fertility of semen diluted by the present new diluent were 74.0 and 55.9 percent in the first and second week respectively. These were fairly higher than those in the previous diluent.

Animal husbandry [ 640 ]

eng

広島大学水畜産学部

[ISSN] 0440-8756

[NCID] AN00213563

[NAID] 40003288821