絶食がめん羊の第1胃内容および血中遊離アミノ酸に及ぼす影響

Effects of Starvation on Rumen Liquid and Amino Acids of Blood Serum in Sheep

Sasaki Masao

Yamatani Yoji

Otani Isao

広島大学水畜産学部紀要

Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University

めん羊2頭を8日間絶食させ，絶食が第1胃内容液および血中遊離アミノ酸に及ぼす影響を検討した.
1. 絶食により胃内容液諸成分はVFA，アンモニア，全窒素および全糖とも経時的に減少した.胃内pHは絶食により上昇し，8日後にはpH8.0に近ずいた.
2. 遊離アミノ酸総量は，血液中では8日間の絶食によっても減少しなかったが，胃内上澄液では絶食4日後にすでに絶食前値の1/10以下となった.血中遊離アミノ酸組成は，絶食により，リジン，グリシンが増加し，グルタミン酸，アラニンが減少した.胃内上澄液では，グルタミン酸，バリン，ロイシンの割合が増加し，リジン，アスパラギン酸，アラニンの割合が減少した.
3. 不可欠アミノ酸の可欠アミノ酸に対する比(E:N Ratio)は絶食により血液で0.87から1.15へと増加したが，胃内上澄液では0.71から0.75となり，有意な変化は認められなかった.

The study，in which two sheep were put on fast for eight days was carried out in order to investigate the effects of starvation on rumen liquid and amino acids of the blood serum.

1) The constituents of rumen liquid-VFA，ammonia，total nitrogen and total sugar contents，all decreased rapidly as the starvation intensified. The pH value of the rumen liquid went up approximately to 8.0 on the eighth day of the fasting.

2) The level of total free amino acids of blood serum didn't vary significantly throughout the starvation. That of rumen liquid，on the other hand， decreased rapidly and resulted in a tenth of the value of pre-experimental period on the fourth day of the fasting. The ratio of lysine and glycine increased but that of glutamic acid and alanine decreased in the blood serum as the effect of the starvation, while the ratio of glutamic acid, valine and isoleucine increased and that of lysine, aspartic acid and alanine decreased in rumen liquid.

3) As the result of the eight-day fasting the essential to non-essential amino acid ratio increased from 0.87 to 1.15 in the blood serum. However, no significant change of the ratio was observed in rumen liquid.

Animal husbandry [ 640 ]

jpn

広島大学水畜産学部

[ISSN] 0440-8756

[NCID] AN00213563

[NAID] 40018463824