Studies on Carotenoprotein in Aquatic Animals : I. Distribution of Carotenoprotein in Exoskeleton of Crayfish (Cambarus clarkii)

広島大学水畜産学部紀要 Volume 10 Issue 2 Page 61-71 published_at 1971-12-25
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Title ( eng )
Studies on Carotenoprotein in Aquatic Animals : I. Distribution of Carotenoprotein in Exoskeleton of Crayfish (Cambarus clarkii)
Title ( jpn )
水産動物のカロチノプロテインに関する研究 : I. アメリカザリガニ甲殻におけるカロチノプロテインの分布
Creator
Nakagawa Heisuke
Kayama Mitsu
Asakawa Suezo
Source Title
広島大学水畜産学部紀要
Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University
Volume 10
Issue 2
Start Page 61
End Page 71
Abstract
1. 0.6M硫安を用いてアメリカザリガニ甲殻より赤,紫,青の三種のカロチノプロテイン(以下CPと略記)を得た.精製後の可視部の最大吸収波長は紫CP615mμ,青CP655mμであった,赤CPの最大吸収は前二者に比較して不明瞭であるが,475mμ付近にわずかな吸収を示した.

2. 甲殻における三穫のCPの分布は部分によって異なり,尾部,頭胸部等の青い甲殻は青CP及び紫CPを多く含み,赤い甲殻では赤CPの占める割合が青い甲殻よりも大きいことを明らかにした.

3. CPを硫安水溶液で抽出後なお赤い色が甲殻に残ることから,甲殻の色はCPのみによるものではなく,非結合型のカロチノイドとCPとの共存によるものと考察した.
1. The blue and the purple carotenoproteins with absorption maxima at 655 and 615 mµ in 0.05 M phosphate buffer at pH 7.3 were obtained from the exoskeleton of the freshwater crayfish (Cambarus clarkii). Besides the above carotenoproteins, the red carotenoprotein which revealed a weak absorption around 475 mµ in visual region was separated in the exoskeleton.

2. Three kinds of carotenoproteins were distributed in various colored exoskeleton in different proportions. In the claws and limbs, the red carotenoprotein was found in somewhat larger degree than in the bluish carapace.

3. A fair amount of carotenoid-pigment remained in the exoskeleton after extraction by aqueous solution. This suggested the coexistence of carotenoproteins and unbound carotenoids in the pigmented exoskeleton.
NDC
Biology [ 460 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
広島大学水畜産学部
Date of Issued 1971-12-25
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0440-8756
[NCID] AN00213563
[NAID] 40018463797