Studies on Rainbow Trout Egg (Salmo gairdnerii irideus) : III. Determination of Lipid Composition of Oil Globule and Lipoprotein

虹鱒卵の生化学的研究 : III. 油球及びリポ蛋白質の脂質組成について

Nakagawa Heisuke

Tsuchiya Yasuhiko

広島大学水畜産学部紀要

Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University

1. 卵黄を遠心分離して油球,低比重画分及び高比重画分(卵黄球)にわけ,それぞれの脂質の分析を行なった.

2. 油球は主にトリグリセライドから成り,他にコレステロール,そのエステル及び遊離の脂肪酸を認めた.リン脂質を全く含まないがカロチノイドに富む.

3. 低比重画分はエーテル処理により,エーテル可溶部と塩溶液可溶部を生じ,前者はトリグリセライド,遊離脂肪酸,コレステロール,そのエステル,リン脂質及びわずかながらカロチノイドを含む.後者は電気泳動によりリポ蛋白質のほか二成分の蛋白質より構成されていることを認めた.

4. 高比重画分の脂質,即ち第1報で報告したリポ蛋白質の脂質は約50%がリン脂質で,他にトリグリセライド,遊離脂肪酸,コレステロール及びそのエステルを含む.リン脂質はレシチン,セファリン,スフィンゴミエリン,リゾレシチンで構成される.

5. それぞれの画分の脂肪酸分析の結果,各画分で脂肪酸組成に相違のあることを認めた.

1. The low density fraction of the rainbow trout eggs which was obtained by centrifugation was separated into the partially defatted lipoprotein and its lipid moiety by ether treatment. This lipoprotein was divided into three components by electrophoresis. The slowest component in mobility was a lipoprotein similar to the slowest component of high density fraction previously reported.

2. The oil globule, characteristically rich in carotenoid and lacking in phosphorus, comprised mainly triglycerides, a small amount of cholesterol, cholesterol esters and a fairly amount of higher fatty acids.

3. The lipid of low density fraction contained triglycerides, cholesterol, cholesterol esters, higher fatty acids, phospholipids and trace amount of carotenoids.

4. The lipid moiety of high density lipoprotein consisted of about 50 per cent of phospholipids, in which lecithin, cephalin, sphingomyelin and lysolecithin were contained. The rest were triglycerides, cholesterol, its esters and higher fatty acids.

5. The fatty acid composition of the oil globule, the lipids of low density fraction and the lipids of high density fraction of the fish egg were determined.

Zoology [ 480 ]

eng

広島大学水畜産学部

[ISSN] 0440-8756

[NCID] AN00213563

[NAID] 40018463850