Microhomology-mediated end-joining-dependent integration of donor DNA in cells using TALENs and CRISPR/Cas9

細胞におけるTALENやCRISPR/Cas9を用いたドナーDNAのマイクロホモロジー媒介末端結合依存的な挿入

Nakade Shouta

Biology [ 460 ]

eng

・Shota Nakade, Takuya Tsubota, Yuto Sakane, Satoshi Kume, Naoaki Sakamoto, Masanobu Obara, Takaaki Daimon, Hideki Sezutsu, Takashi Yamamoto, Tetsushi Sakuma and Ken-ichi T. Suzuki; Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9; Nature Communications, 5, 5560 (2014). (doi: 10.1038/ncomms6560) references

・Yu Hisano, Tetsushi Sakuma, Shota Nakade, Rie Ohga, Satoshi Ota, Hitoshi Okamoto, Takashi Yamamoto and Atsuo Kawahara; Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish; Scientific Reports, 5, 8841 (2015). (doi: 10.1038/srep08841) references

・Tetsushi Sakuma, Shota Nakade, Yuto Sakane, Ken-Ichi T. Suzuki and Takashi Yamamoto; MMEJ-assisted gene knock-in using TALENs and CRISPR/Cas9 with the PITCh systems; Nature protocols, 11(1), 118-133 (2016). (doi: 10.1038/nprot.2015.140) references

・Shota Nakade, Tetsushi Sakuma, Yuto Sakane, Yoshihiro Hara, Atsushi Kurabayashi, Keiko Kashiwagi, Akihiko Kashiwagi, Takashi Yamamoto and Masanobu Obara; Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs; In Vitro Cellular & Developmental Biology-Animal, 51(9), 879-884 (2015). (doi: 10.1007/s11626-015-9912-0) references

[DOI] http://doi.org/10.1038/ncomms6560 references

[DOI] http://doi.org/10.1038/srep08841 references

[DOI] http://doi.org/10.1038/nprot.2015.140 references

[DOI] http://doi.org/10.1007/s11626-015-9912-0 references

広島大学