Identification of a Novel Vanadium-binding Protein by EST Analysis on the Most Vanadium-rich Ascidian, Ascidia gemmata
Mar Biotechnol 14 巻 2 号
143-154 頁
2012-04 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Identification of a Novel Vanadium-binding Protein by EST Analysis on the Most Vanadium-rich Ascidian, Ascidia gemmata
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| 作成者 |
Samino Setijono
Michibata Hitoshi
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| 収録物名 |
Mar Biotechnol
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| 巻 | 14 |
| 号 | 2 |
| 開始ページ | 143 |
| 終了ページ | 154 |
| 抄録 |
Ascidians are known to accumulate extremely high levels of vanadium in their blood cells (up to 350 mM). The branchial sac and the intestine are thought to be the first tissues to contact the outer environment and absorb vanadium ions. The concentration of vanadium in the branchial sac and the intestine of the most vanadium-rich ascidian A. gemmata were determined to be 32.4 mM and 11.9 mM, respectively. Using an expressed sequence tag (EST) analysis of a cDNA library from the intestine of A. gemmata, we determined 960 ESTs and found 55 clones of metal-related gene orthologs, 6 redox-related orthologs, and 18 membrane transporter orthologs. Among them, 2 genes, exhibited significant similarity to the vanadium-binding proteins of other vanadium-rich ascidian species, were designated AgVanabin1 and AgVanabin2. Immobilized metal ion affinity chromatography revealed that recombinant AgVanabin1 bound to metal ions with an increasing affinity for Cu(II) > Zn(II) > Co(II), and AgVanabin2 bound to metal ions with an increasing affinity for Cu(II) > Fe(III) > V(IV). To examine the use of AgVanabins for a metal absorption system, we constructed Escherichia coli strains that expressed AgVanabin1 or AgVanabin2 fused to maltose binding protein and secreted into the periplasmic space. We found that the strain expressing AgVanabin2 accumulated about 13.5 times more Cu(II) ions than the control TB1 strain. Significant accumulation of vanadium was also observed in the AgVanabin2-expressing strain as seen by a 1.5-fold increase.
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| 著者キーワード |
vanadium
vanabin
metal accumulation
ascidian
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| 内容記述 |
Financial support was provided in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (#20570070 and #21570077).
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Springer-Verlag
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| 発行日 | 2012-04 |
| 権利情報 |
Copyright (c) Springer Science+Business Media, LLC 2011
The final publication is available at Springer via http://dx.doi.org/10.1007/s10126-011-9396-1.
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 1436-2228
[NCID] AA11357643
[DOI] 10.1007/s10126-011-9396-1
[DOI] http://doi.org/10.1007/s10126-011-9396-1
～の異版である
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