Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells
Hiroshima Journal of Medical Sciences Volume 33 Issue 2
Page 279-285
published_at 1984-06
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| File | |
| Title ( eng ) |
Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells
|
| Title ( jpn ) |
ルシフェラーゼ試薬を用いる培養細胞の ATP 測定法
|
| Creator |
Kiyotani Katsuhiro
Kanamoto Yasuo
Matsuo Yoshiyasu
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 33 |
| Issue | 2 |
| Start Page | 279 |
| End Page | 285 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
A method for determining intracellular content of adenosine triphosphate (ATP) in the cultured cells was investigated with a firefly luciferase reagent, and a micro assay method was established. The method minimized the size of both samples and luciferase reagent employed.
The per cell amount of intracellular ATP of HeLa 229 and McCoy cells was estimated to be 7.20×10-11 M arid 8.74×10-11 M, respectively. The method could be applied to a direct measurement of the ATP content in cultured cells grown in each well of a 96-well micro tissue culture plate. |
| Keywords |
Firefly luciferase
ATP
HeLa 229 cells
McCoy cells
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1984-06 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6480392
|
