A New Method to Detect Response of Lymphocytes to Alla-antigens
Hiroshima Journal of Medical Sciences Volume 45 Issue 3
Page 99-103
published_at 1996-09
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Title ( eng ) |
A New Method to Detect Response of Lymphocytes to Alla-antigens
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Creator |
Okimoto Tatsuya
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 45 |
Issue | 3 |
Start Page | 99 |
End Page | 103 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
The mixed lymphocyte reaction (MLR) is the most commonly used method of investigating the immunological response of lymphocytes. However, this conventional technique requires the use of a radioisotope and is time consuming. Furthermore, there is a possibility that MLR does not reflect the response of each lymphocyte subset individually. The purpose of this study is to determine whether detection of IL-2 mRNA by reverse transcription polymerase chain reaction (RT-PCR) can be used as a new method of studying a precise response of PEL to allo-antigens. The study was conducted using lymphocytes from healthy volunteers, a renal transplant recipient who exhibited donor specific MLR unresponsiveness, the donor and the recipient's father. A kinetics study revealed that the expression of IL-2 mRNA in the MLC reached its maximum at 24-48 h. Lymphocytes from a renal transplant recipient also exhibited the same IL-2 mRNA expression pattern in response to stimulation with PEL from a non-related 3rd party but not to that from the donor. These data suggest that the expression level of IL-2 mRNA in MLC measured by RT-PCR at 24-48 h provides accurate response of PEL to allo-antigens.
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Keywords |
MLC
IL-2 mRNA
RT-PCR
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University Medical Press
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Date of Issued | 1996-09 |
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
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