Dysfunction of Immune System and Induction of Autoantibodies to Liver Antigens by Neonatal Thymectomy in Mice
Hiroshima Journal of Medical Sciences Volume 45 Issue 3
Page 75-83
published_at 1996-09
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| File | |
| Title ( eng ) |
Dysfunction of Immune System and Induction of Autoantibodies to Liver Antigens by Neonatal Thymectomy in Mice
|
| Creator |
Myozaki Mitsuru
Wang Mei Qi
Yoshida Yasuhiro
Liu Jia Nuo
Kuroda Etsushi
Yamashita Uki
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 45 |
| Issue | 3 |
| Start Page | 75 |
| End Page | 83 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
We examined the development of autoantibodies to liver proteins and hepatitis in BALB/c mice thymectomized 2 days after birth and attempted to characterize the immune function of these mice. Autoantibodies to crude liver proteins detected by ELISA were found in 21(84%) of 25 mice thymectomized 2 days after birth. In these mice, sera of 11 animals showed reactivity with both liver specific proteins (LSP) and the second fraction of crude liver proteins; sera of 3 mice showed reactivity with only the second fraction but no sera showed reactivity with only LSP. By Western-immunoblotting, sera of BALB/c mice which showed high autoantibody level to liver proteins detected a strong band around 150kD in the second fraction of crude liver proteins. Still more, hepatic inflammation; mononuclear cell infiltration in the portal area, was induced in mice with apparently high autoantibody level to crude liver proteins. These results in BALB/c mice corresponded with our previous reports which employed C3H/HeN mice.
Next, we examined immune functions of mice thymectomized 2 days after birth. In thymectomized mice, the proportion of Thy-1, L3T4 and Lyt-2 positive cells (T cells) decreased and the proportion of B220 positive cells (B cells) increased. The proliferative response of lymph node lymphocytes cultured with mitomycin C-treated syngeneic spleen cells was lower, and the total IgG level in the sera was higher when compared with control normal mice. Anti-nuclear antibody (ANA) also appeared in the sera of thymectomized mice 2 days after birth. All these results suggest that the dysfunction of T cell and polyclonal activation of B cell were induced in neonatally thymectomized mice and resulted in the production of ANA and autoantibodies to liver proteins. |
| Keywords |
Neonatal thymectomy
Immune dysfunction
Autoantibody to liver
|
| Descriptions |
This work was supported in part from a grant-in-aid of Fukuyama Transporting Shibuya Longevity and Health Foundation.
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 1996-09 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
|
