X-rays Induce Dose-dependent and Cell Cycle-independent Accumulation of p21sdi1/WAF1
Hiroshima Journal of Medical Sciences Volume 50 Issue 1
Page 1-7
published_at 2001-03
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| File | |
| Title ( eng ) |
X-rays Induce Dose-dependent and Cell Cycle-independent Accumulation of p21sdi1/WAF1
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| Creator |
Tsuyama Naohiro
Ide Toshinori
Noda Asao
Iwamoto Keisuke S.
Mizuno Terumi
Kyoizumi Seishi
Seyama Toshio
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| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 50 |
| Issue | 1 |
| Start Page | 1 |
| End Page | 7 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Cell cycle arrest at the G1 checkpoint is governed by a function ofwild-typep53. We assessed the behavior of the sdi1 gene, which codes for a 21kDa potent inhibitor of cdk/cyclins, after X-irradiation. X-irradiation induced sdi1 mRNA accumulation and G1 arrest only in cells possessing wild-type p53. Elevation of p21sdi1/WAF1 was preceded by p53 accumulation, which occurred despite p53 mRNA constancy in normal cells growing in the log phase. The quantity of accumulated p53 and p21sdi1/WAF1 was radiation dose dependent. A decrease in the S phase cell population in normal cells observed after irradiation reached a minimum at less-than-maximum levels of p53 and p21sdi1/WAF1 Furthermore, an accumulation of p53 and p21sdi1/WAF1 was also observed when cells were synchronized in the G0, G1 and S phase and X-irradiated. These results indicated that an X-ray induced p53 and p21sdi1/WAF1 accumulation mechanism exists throughout the cell cycle, and that the signal strength induced by X-irradiation is dose-dependent.
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| Keywords |
X-irradiation
p21sdi1/WAF1
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| Descriptions |
This publication is based on research performed at the Radiation Effects Research Foundation (RERF), Hiroshima and Nagasaki, Japan and is supported in part by Grants-in-Aid (S7-l) for Cancer Research from the Ministry of Health and Welfare.
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| NDC |
Medical sciences [ 490 ]
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| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 2001-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
|
