RNA POLYMERASES AND THEIR STIMULATING FACTOR FROM LEUKEMIA L1210 ASCITES CELLS
Hiroshima Journal of Medical Sciences Volume 30 Issue 2
Page 93-97
published_at 1981-06-10
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| File | |
| Title ( eng ) |
RNA POLYMERASES AND THEIR STIMULATING FACTOR FROM LEUKEMIA L1210 ASCITES CELLS
|
| Title ( jpn ) |
Leukemia L1210 腹水細胞よりの RNA ポリメラーゼとその促進因子について
|
| Creator |
Tomioka Haruaki
Saito Hajime
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 30 |
| Issue | 2 |
| Start Page | 93 |
| End Page | 97 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
RNA polymerases I and II were partially purified from L1210 ascites cells by sonic disruption, batchwise treatment with diethylaminoethyl (DEAE) cellulose, ammonium sulfate-fractionation, and subsequent linear KCl gradient chromatograpy on DEAE cellulose column. The RNA polymerases from L1210 cells showed similar enzymatic properties as in cases of Ehrlich carcinoma-RNA polymerases. RNA polymerase-stimulating factor was obtained by carboxymethyl (CM) cellulose column chromatography from L1210 cell lysate. The factor enhanced both of RNA polymerases I and II when native calf thymus DNA was used as a template, although it failed to casuse any stimulating effect when poly dAT was employed as a template DNA.
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| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1981-06-10 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
|
