5β-CHOLESTANE-3α, 27-DIOL: NAD+ OXIDOREDUCTASE AND 3α-HYDROXY-5β-CHOLESTAN-27-AL: NAD+ OXIDOREDUCTASE IN RAT LIVER EXTRACT
Hiroshima Journal of Medical Sciences Volume 30 Issue 1
Page 1-7
published_at 1981-03-10
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| File | |
| Title ( eng ) |
5β-CHOLESTANE-3α, 27-DIOL: NAD+ OXIDOREDUCTASE AND 3α-HYDROXY-5β-CHOLESTAN-27-AL: NAD+ OXIDOREDUCTASE IN RAT LIVER EXTRACT
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| Title ( jpn ) |
白ネズミ肝臓抽出液中の 5β-cholestane-3α, 27-diol: NAD+ 酸化還元酵素ならびに 3α-hydroxy-5β-cholestan-27-al: NAD+ 酸化還元酵素
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| Creator |
Tsukiai Satsuki
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| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 30 |
| Issue | 1 |
| Start Page | 1 |
| End Page | 7 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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| Abstract |
Using rat liver alcohol:NAD+ oxidoreductase and aldehyde:NAD+ oxidoreductase, the following facts were found: 1) 3α-hydroxy-5β-cholestan-27-al was reduced to 5β-cholestane-3α, 27-diol by liver alcohol: NAD+ oxidoreductase. 2) The optimum pH was pH 7.0 with potassium phosphate buffer and pH 8.5 with Tris buffer. 3) The Km value of the substrate to the enzyme was 14.3 μM. 4) 3α-Hydroxy-5β-cholestan-27- al was oxidized to 3α-hydroxy-5β-cholestan-27-oic acid by liver aldehyde: NAD+ oxidoreductase. 5) The optimum pH was pH 8.5 with Tris buffer. 6) The Km value of the substrate to the enzyme was 26.3 μM.
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| NDC |
Medical sciences [ 490 ]
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| Language |
eng
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| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
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| Date of Issued | 1981-03-10 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
|
