TABLE OF CONTENTS
PREFACE / p1
Chapter I Expression of a Gene for Human Protein:Genetic Design of a Gene for Human Metallothionein II and Its Expression as an Active Fusion Protein in Escherichia coli / p9
　1.Introduction / p10
　2.Materials and methods / p11
　3.Results / p15
　4.Discussion / p24
　5.Summary / p27
　6.References / p28
Chapter II Production and Purification of Monoamine Oxidase:Production, Purification and Characterization of a new type of Monoamine Oxidase containing Cu from Klebsiella aerogenes / p32
　1.Introduction / p33
　2.Materials and methods / p35
　3.Results / p39
　4.Discussion / p51
　5.Summary / p54
　6.References / p55
Chapter III Structure and Function of Pullulanase:Role of Lipid Modification on a Starch-debranching Enzyme,Klebsiella Pullulanase / p60
　1.Introduction / p61
　2.Materials and methods / p63
　3.Results / p65
　4.Discussion / p75
　5.Summary / p77
　6.References / p78
Chapter IV Functinal Development of Pullulanase:Random mutagenesis of Pullulanase from Klebsilella aerogenes for Studies of the Structure and Function of the Enzyme / p80
　1.Introduction / p81
　2.Materials and methods / p83
　3.Results / p85
　4.Discussion / p96
　5.Summary / p100
　6.References / p101
GENERAL DISCUSSION(CONCLUSION) / p107
REFERRENCE PUBLICATIONS / p109
RELATED PUBLICATIONS / p111
ACKNOWLEDGEMENTS / p113