Studies on bombardment-mediated transformation of pollen cells in angiosperms
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| File | |
| Title ( eng ) |
Studies on bombardment-mediated transformation of pollen cells in angiosperms
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| Title ( jpn ) |
パーティクルガン法による被子植物花粉の形質転換に関する研究
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| Creator |
Nishihara Masahiro
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| Descriptions |
CONTENTS
INTRODUCTION / p1 CHAPTER I GENE DELIVERY AND EXPRESSION IN POLLEN CELLS BY PARTICLE BOMBARDMENT / p4 Section1 Transient expression of the β-glucuronidase gene in pollen by particle bombardment / p4 Section2 Optimization of bombardment conditions for pollen cells / p11 Section3 Comparison of promoter activities of pollen-expressed genes in pollen of mono- and dicot plants / p16 Section4 Cytological observation of GUS-expressing pollen of lily / p23 CHAPTER II TRANSGENIC HAPLOID PLANTS PRODUCED BY BOMBARDMENT-MEDIATED TRANSFORMATION / p29 Section1 Regeneration of pollen-derived transgenic haploid plants of Nicotiana rustica / p29 Section2 Analyses of transgenic haploid plants of Nicotiana rustica / p35 CONCLUSIONS / p44 ACKNOWLEDGMENTS / p46 REFERENCES / p48 LIST OF PUBLICATIONS / p57 |
| NDC |
Botany [ 470 ]
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| Language |
eng
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| Resource Type | doctoral thesis |
| Rights |
Copyright(c) by Author
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| Publish Type | Not Applicable (or Unknown) |
| Access Rights | open access |
| Source Identifier |
・Nishihara, M., Ito, M., Tanaka, I., Kyo, M., Ono, K., Irifune, K., and Morikawa, H. (1993) Expression of the β-glucuronidase gene in pollen of lily (Lilium longiflorum), tobacco (Nicotiana tabacum), Nicotiana rustica, and peony (Paeonia lactiflora) by particle bombardment. Plant Physiol. 102: 357-361.
references
・Nishihara, M., Seki, M., Kyo, M., Irifune, K., and Morikawa, H. Transgenic haploid plants of Nicotiana rustica produced by bombardment-mediated transformation. Transgenic Res. (in press).
references
[DOI] http://dx.doi.org/10.1104/pp.102.2.357
references
[DOI] http://dx.doi.org/10.1007/BF01972531
references
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| Dissertation Number | 甲第1300号 |
| Degree Name | |
| Date of Granted | 1994-11-28 |
| Degree Grantors |
広島大学
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