Studies on bombardment-mediated transformation of pollen cells in angiosperms

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Title ( eng )
Studies on bombardment-mediated transformation of pollen cells in angiosperms
Title ( jpn )
パーティクルガン法による被子植物花粉の形質転換に関する研究
Creator
Nishihara Masahiro
Descriptions
CONTENTS
INTRODUCTION / p1
CHAPTER I GENE DELIVERY AND EXPRESSION IN POLLEN CELLS BY PARTICLE BOMBARDMENT / p4
 Section1 Transient expression of the β-glucuronidase gene in pollen by particle bombardment / p4
 Section2 Optimization of bombardment conditions for pollen cells / p11
 Section3 Comparison of promoter activities of pollen-expressed genes in pollen of mono- and dicot plants / p16
 Section4 Cytological observation of GUS-expressing pollen of lily / p23
CHAPTER II TRANSGENIC HAPLOID PLANTS PRODUCED BY BOMBARDMENT-MEDIATED TRANSFORMATION / p29
 Section1 Regeneration of pollen-derived transgenic haploid plants of Nicotiana rustica / p29
 Section2 Analyses of transgenic haploid plants of Nicotiana rustica / p35
CONCLUSIONS / p44
ACKNOWLEDGMENTS / p46
REFERENCES / p48
LIST OF PUBLICATIONS / p57
NDC
Botany [ 470 ]
Language
eng
Resource Type doctoral thesis
Rights
Copyright(c) by Author
Publish Type Not Applicable (or Unknown)
Access Rights open access
Source Identifier
・Nishihara, M., Ito, M., Tanaka, I., Kyo, M., Ono, K., Irifune, K., and Morikawa, H. (1993) Expression of the β-glucuronidase gene in pollen of lily (Lilium longiflorum), tobacco (Nicotiana tabacum), Nicotiana rustica, and peony (Paeonia lactiflora) by particle bombardment. Plant Physiol. 102: 357-361. references
・Nishihara, M., Seki, M., Kyo, M., Irifune, K., and Morikawa, H. Transgenic haploid plants of Nicotiana rustica produced by bombardment-mediated transformation. Transgenic Res. (in press). references
[DOI] http://dx.doi.org/10.1104/pp.102.2.357 references
[DOI] http://dx.doi.org/10.1007/BF01972531 references
Dissertation Number 甲第1300号
Degree Name
Date of Granted 1994-11-28
Degree Grantors
広島大学