Differentiation of a hepatic phenotype after heterotropic transplantation of heart, kidney, brain, and skin tissues into liver in F344 rats

Watanabe Hiromitsu

Ochiya Takahiro

Ueda Shinobu

Kominami Yoko

Gon Rina

Nishiki Masayo

Hayashi Masaomi

Sasaki Atsushi

Shiraishi Miho

Kashimoto Naoki

Myojin Yuki

Kamiya Kenji

Biochemical and Biophysical Research Communications

While organ-specific stem cells with roles in tissue injury repair have been documented, their pathogenic significance in diseases and the factors potentially responsible for their activation remain largely unclear. In the present study, heart, kidney, brain, and skin samples from F344 transgenic rats carrying the GFP gene were transplanted into normal F344 rat liver one day after an intraperitoneal injection (i.p.) of carbon tetrachloride (CCl4) to test their differentiation capacity. The transplantation was carried out by female donors to male recipients, and vice versa. One week after transplantation, GFP antigen-positive cells with phenotypic characteristics of hepatocytes were noted. After two weeks, their extent increased, and at 4 weeks, large areas of strongly GFP-stained cells developed. All recipient livers had GFP antigen-positive hepatocyte cells. PCR analysis coupled with laser capture micro-dissection (LCM) revealed those cells to contain GFP DNA. Thus, our results indicate that tissue stem cells have multipotential ability, differentiating into hepatocytes when transplanted into an injured liver.

Differentiation

Hepatocyte

Transplantation

Stem cells

Liver

GFP

Transgenic rat

CCl4

Medical sciences [ 490 ]

eng

Elsevier

Copyright (c) 2007 Elsevier Inc. All rights reserved.

[ISSN] 0006-291X

[DOI] 10.1016/j.bbrc.2006.12.236

[NCID] AA00564395

[DOI] http://dx.doi.org/10.1016/j.bbrc.2006.12.236