Slow molecular dynamics close to crystal surfaces during crystallization of a protein lysozyme studied by fluorescence correlation spectroscopy
Journal of Chemical Physics Volume 133 Issue 9
Page 095103-1-095103-9
published_at 2010
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Title ( eng ) |
Slow molecular dynamics close to crystal surfaces during crystallization of a protein lysozyme studied by fluorescence correlation spectroscopy
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Creator | |
Source Title |
Journal of Chemical Physics
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Volume | 133 |
Issue | 9 |
Start Page | 095103-1 |
End Page | 095103-9 |
Abstract |
Fluorescence correlation spectroscopy FCS was applied to the crystallization processes of egg-white lysozyme. Utilizing FCS's high spatial resolution of about the laser wavelength used, the molecular dynamics close to crystal surfaces was investigated for both tetragonal single crystals and needlelike spherulites. When the FCS measurement was done at the point closer than 1 m to the surface of a tetragonal single crystal, the relaxation time became several times longer than that in bulk solution, but the fluorescence intensity thus concentration was similar to that observed in bulk solution. On the other hand, the peculiar slow dynamics a few orders of magnitude slower than that in bulk solution of concentrated liquid states of the lysozyme molecules was observed in needlelike spherulites. We suggested that these observations could be explained by the formation of softly connected aggregates accumulating around the needlelike crystals, which could cause the instability of the crystal growth and thus the formation of spherulites. These aggregates gradually disappeared as the crystallization further proceeded. After the disappearance of the aggregates, the spherulites started to mature.
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Keywords |
crystallisation
fluorescence
optical correlation
proteins
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NDC |
Chemistry [ 430 ]
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Language |
eng
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Resource Type | journal article |
Publisher |
The American Institute of Physics
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Date of Issued | 2010 |
Rights |
Copyright (c) 2010 American Institute of Physics.
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Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0021-9606
[DOI] 10.1063/1.3478224
[NCID] AA00694991
[DOI] http://dx.doi.org/10.1063/1.3478224
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