Abscisic acid stimulated ripening and gene expression in berry skins of the Cabernet Sauvignon grape

Functional & Integrative Genomics Volume 10 Issue 3 Page 367-381 published_at 2010
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Title ( eng )
Abscisic acid stimulated ripening and gene expression in berry skins of the Cabernet Sauvignon grape
Creator
Koyama Kazuya
Sadamatsu Keiko
Goto-Yamamoto Nami
Source Title
Functional & Integrative Genomics
Volume 10
Issue 3
Start Page 367
End Page 381
Abstract
We investigated the effect of exogenous abscisic acid (ABA) application on the transcriptome as well as the phenolic profiles in the skins of Vitis vinifera cv. Cabernet Sauvignon grape berries grown on the vine and cultured in vitro. ABA application rapidly induced the accumulation of anthocyanin and flavonol. Correlatively, the structural genes in the phenylpropanoid and flavonoid pathways, their transcriptional regulators, as well as genes considered to be involved in the acylation and transport of anthocyanin into the vacuole, were upregulated by ABA treatment. The Genechip analysis showed that the ABA treatment significantly up- or downregulated a total of 345 and 1,482 transcripts in the skins of berries grown on the vine and cultured in vitro, respectively. Exogenous ABA modulated the transcripts associated with osmotic responses, stress responses, cell wall modification, auxin and ethylene metabolism and responses, in addition to the induction of anthocyanin biosynthetic genes, and reduced those associated with photosynthesis; approximately half of these transcripts were identical to the previously reported ripening-specific genes.
Keywords
Abscisic acid (ABA)
Ripening
Anthocyanin biosynthesis
Affymetrix oligonucleotide array
Berry skins
Vitis vinifera
NDC
Botany [ 470 ]
Language
eng
Resource Type journal article
Publisher
Springer Heidelberg
Date of Issued 2010
Rights
Copyright (c) 2010 Springer
Publish Type Author’s Original
Access Rights open access
Source Identifier
The original publication is available at www.springerlink.com
[ISSN] 1438-793X
[DOI] 10.1007/s10142-009-0145-8
[NCID] AA11461215
[DOI] http://dx.doi.org/10.1007/s10142-009-0145-8