Continuous-Flow ATP amplification system on a chip
Sensors and Actuators B: Chemical Volume 142 Issue 1
Page 118-122
published_at 2009-10-12
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| File | |
| Title ( eng ) |
Continuous-Flow ATP amplification system on a chip
|
| Creator |
Satoh Tetsuya
Shinoda Yasuharu
Tokonami Shiho
Hirota Ryuichi
|
| Source Title |
Sensors and Actuators B: Chemical
|
| Volume | 142 |
| Issue | 1 |
| Start Page | 118 |
| End Page | 122 |
| Abstract |
In this study, we constructed a novel microfluidic device for continuous-flow ATP amplification, using the SU-8:PDMS method. Sepharose beads immobilized with adenylate kinase and pyruvate kinase was packed into a microfluidic chamber to form lamination layer. Dry film type SU-8 was suitable to form a very thick mold for beads column reactor and its dam structure. A good correlation between amplified luminescence and initial ATP concentration was observed in this system. The gradient of amplification when performing six cycles of continuous-flow ATP amplification was 1.72(N).
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| Keywords |
Bioluminescence assay
ATP amplification
Continuous-flow system
PDMS chip
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| NDC |
Chemistry [ 430 ]
|
| Language |
eng
|
| Resource Type | journal article |
| Publisher |
Elsevier Science Sa
|
| Date of Issued | 2009-10-12 |
| Rights |
Copyright (c) 2009 Elsevier B.V.
|
| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0925-4005
[DOI] 10.1016/j.snb.2009.08.020
[NCID] AA1078104X
[DOI] http://dx.doi.org/10.1016/j.snb.2009.08.020
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