Detection of living Salmonella cells using bioluminescence
Biotechnology Letters Volume 31 Issue 5
Page 737-741
published_at 2009
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| File | |
| Title ( eng ) |
Detection of living Salmonella cells using bioluminescence
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| Creator |
Urata Masaaki
Iwata Rei
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| Source Title |
Biotechnology Letters
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| Volume | 31 |
| Issue | 5 |
| Start Page | 737 |
| End Page | 741 |
| Abstract |
ATP-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for Salmonella enteritidis detection. In this combination method, the Salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the ATP released from the cells was measured using mutant luciferase. This method resulted in approximately 1,000 times higher sensitivity in the detection of Salmonella (i.e. 10(3) c.f.u./ml) compared to immunochromatographic lateral flow assay.
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| Keywords |
ATP assay
Immunochromatographic lateral flow assay
Mutant firefly luciferase
Salmonella enteritidis
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| NDC |
Chemical technology [ 570 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Springer
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| Date of Issued | 2009 |
| Rights |
Copyright (c) 2009 Springer-Verlag. "The original publication is available at www.springerlink.com"
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0141-5492
[DOI] 10.1007/s10529-009-9924-5
[NCID] AA00110183
[DOI] http://dx.doi.org/10.1007/s10529-009-9924-5
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