Enhancement of Osteogenesis by Concanavalin A in Human Bone Marrow Mesenchymal Stem Cell Cultures

The International Journal of Artificial Organs 31 巻 8 号 708-715 頁 2008-08 発行
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タイトル ( eng )
Enhancement of Osteogenesis by Concanavalin A in Human Bone Marrow Mesenchymal Stem Cell Cultures
作成者
Sekiya Kensuke
Nishimura Masahiro
Suehiro Fumio
Nishimura Haruki
Hamada Taizo
Kato Yukio
収録物名
The International Journal of Artificial Organs
31
8
開始ページ 708
終了ページ 715
抄録
This study investigates concanavalin A (ConA) as a novel factor that may enhance osteogenesis of mesenchymal stem cells (MSCs) in vitro. Various factors have been studied as promoting factors for MSC osteogenesis in vivo and in vitro. However, their safety, effectiveness, and cost may not be ideal. So, human MSCs were cultured in osteogenic medium in the presence or absence of ConA. We used calcium assays to compare the effects of ConA and BMP-2 on MSC calcification. Also enzyme-linked immunosorbent assay (ELISA) and quantitative PCR were used to evaluate the expression levels of bone specific markers. ConA was observed to enhance the calcification and this effect was comparable to that of BMP-2. Combination of ConA and BMP-2 further enhanced the calcification slightly but significantly. ConA also increased osteocalcin and BMP-2 protein levels in the medium of MSC cultures. Furthermore, ConA increased osteocalcin, RUNX2, BMP-2, and BMP-4 mRNA expression levels. However, gene expression pattern of MSCs stimulated by ConA was different from that observed with BMP-2. These results, taken together, suggest that ConA and BMP-2 enhance MSC osteogenesis via different pathways. The ConA-induced bone formation in MSC cultures may be useful in regenerative medicine or tissue engineering in clinical studies and in basic research on bone formation.
著者キーワード
Concanavalin A
Mesenchymal stem cells
Calcification
Osteogenesis
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Wichtig Editore
発行日 2008-08
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0391-3988
[NCID] AA00679932