Purification of a Lethal Toxin of Edwardsiella tarda
魚病研究 Volume 31 Issue 4
Page 203-207
published_at 1996-12
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| File | |
| Title ( eng ) |
Purification of a Lethal Toxin of Edwardsiella tarda
|
| Creator |
Suprapto Hari
Hara Takaaki
Muroga Kiyokuni
|
| Source Title |
魚病研究
Fish Pathology
|
| Volume | 31 |
| Issue | 4 |
| Start Page | 203 |
| End Page | 207 |
| Abstract |
A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6µg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and imrnunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tardy but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.
|
| Keywords |
Edwardsiella tarda
toxin
extracellular product
intracellular component
Japanese eel
ECP
|
| NDC |
Fishing industry. Fisheries [ 660 ]
|
| Language |
eng
|
| Resource Type | journal article |
| Publisher |
日本魚病学会
|
| Date of Issued | 1996-12 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0388-788X
[NCID] AN00063165
|
