Sodium chloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells

Edwardsiella tarda のHEp-2細胞への付着性に及ぼす培地食塩濃度の影響

Mahmoud Mostafa Mahmoud

Okuda Jun

Nakai Toshihiro

魚病研究

Fish Pathology

異なる食塩濃度の培地で培養したE. tarda 3株のHEp-2細胞への付着性を比較した。生菌数測定, 直接光顕観察のいずれにおいても, FK1051株およびKG8401株は3%食塩濃度の培地で培養すると0%食塩濃度の培地での培養に比べて高い細胞付着性を示したが, SU166株のそれは培地の塩分濃度にかかわらず低かった。これらの細胞付着性と培地食塩濃度との関係は, 赤血球凝集活性とのそれとよく一致した。また, この細胞付着性はN-アセチルノイラミン酸によって阻害された。

The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish.

Edwardsiella tarda

adherence

hemagglutination

HEp-2 cells

N-acetylneuraminic acid

Zoology [ 480 ]

eng

日本魚病学会

Copyright (c) 2007 The Japanese Society of Fish Pathology

[ISSN] 0388-788X

[ISSN] 1881-7335

[DOI] 10.3147/jsfp.41.165

[NCID] AN00063165

[DOI] http://dx.doi.org/10.3147/jsfp.41.165