Detection of antibodies against striped jack nervous necrosis virus (SJNNV) from brood stocks of striped jack.

Nippon Suisan Gakkaishi Volume 58 Issue 12 Page 2351-2356 published_at 1992
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Title ( eng )
Detection of antibodies against striped jack nervous necrosis virus (SJNNV) from brood stocks of striped jack.
Creator
Mushiake Keiichi
Arimoto Misao
Furusawa Toru
Furusawa Iwao
Muroga Kiyokuni
Source Title
Nippon Suisan Gakkaishi
Volume 58
Issue 12
Start Page 2351
End Page 2356
Abstract
It has been indicated that parental spawners were a source of infection of a viral disease, “viral nervous necrosis", in larval striped jack Pseudocaranx dentex. In this study, the prevalence of antibodies against the causative virus (SJNNV) was examined among brood stocks of striped jack by indirect ELISA. Indirect ELISA using purified SJNNV, rabbit anti-SJNNV serum, and enzyme-conjugated goat anti-rabbit IgG antibody was employed for antibody detection from the plasma. The plasma IgM partially purified by ion-exchange chromatography was used for ELISA detection. The antibody to SJNNV was detected at high frequency (65%) in plasma samples collected from brood stocks reared at various facilities regardless of their sex or origin (wild or domestic). This indicates that the virus is prevalent among cultured populations of this fish species.
NDC
Zoology [ 480 ]
Language
eng
Resource Type journal article
Publisher
The Japanese Society of Fisheries Science
Date of Issued 1992
Rights
Copyright (c) The Japanese Society of Fisheries Science
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0021-5392
[ISSN] 1349-998X
[NCID] AN00193422
[URI] http://www.journalarchive.jst.go.jp/english/jnlreference_en.php?cdjournal=suisan1932&cdvol=58&noissue=12&startpage=2351