Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis (SJNNV)
Diseases of Aquatic Organisms Volume 18
Page 103-107
published_at 1994-02-24
アクセス数 : 990 件
ダウンロード数 : 232 件
今月のアクセス数 : 3 件
今月のダウンロード数 : 2 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00025651
File |
dao018p103.pdf
530 KB
種類 :
fulltext
|
Title ( eng ) |
Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis (SJNNV)
|
Creator |
Nishizawa Toyohiko
Mori Koh-ichiro
Furusawa Iwao
Muroga Kiyokuni
|
Source Title |
Diseases of Aquatic Organisms
|
Volume | 18 |
Start Page | 103 |
End Page | 107 |
Abstract |
The polymerase chain reaction (PCR) was used to amplify a portion of the coat protein gene (RNA2) of striped jack nervous necrosis vlrus (SJNNV), the causative agent of viral nervous necrosis (VNN) of larval striped jack Pseudocaranx dentex. Based on the sequence data of SJNNV RNA2, 2 forward (F1 and F2) and 3 reverse (RI. R2 and R3) PCR primers were synthesized and the 5 potential target regions were amplified with a combination of these primers. After reverse transcription of genomic RNA extracted from SJNNV and 25 cycles of PCR amplification, products of the expected size were detected separately on agarose gels stained with ethidium bromide. Southern blot hybridization confirmed that all of the amplified products were specific to cDNA of SJNNV RNA2. Two primer sets, F1-R2 and F2-R3, produced the specified 180 bp and 430 bp products. The PCR system, using the F2-R3 primer set, was able to detect 100 fg of SJNNV RNA after 25 cycles and was also able to efficiently amplify the target region of SJNNV gene in the total nucleic acids extracted from larval striped jack affected with VNN.
|
Keywords |
Viral nervous necrosis
Nodavirus
PCR
|
NDC |
Zoology [ 480 ]
|
Language |
eng
|
Resource Type | journal article |
Publisher |
Inter-Research
|
Date of Issued | 1994-02-24 |
Rights |
Copyright (c) 1994 Inter-Research.
|
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0177-5103
[ISSN] 1616-1580
[DOI] 10.3354/dao018103
[NCID] AA10443976
[DOI] http://dx.doi.org/10.3354/dao018103
|