Immunoexpression of CXC-chemokine and recruitment of polymorphonuclear leukocytes in the rat molar periodontal tissue after topical application of lipopolysaccharide

Miyauchi Mutsumi

Kitagawa Shoji

Hiraoka Masae

Saito Akihisa

Sato Sunao

Kudo Yasusei

Ogawa Ikuko

Takata Takashi

Histochemistry and Cell Biology

Accumulating evidence indicates that an important event in the tissue response in periodontal disease is the recruitment of polymorphonuclear leukocytes (PMN). This study investigated immunohistochemical expression of CXC-chemokines, including macrophage inflammatory protein-2 (MIP-2) and cytokine-induced neutrophil chemoattractant-2 (CINC-2) in rat periodontal tissue at 1 and 3 hours and 1, 2, 3 and 7 days after topical application of lipopolysaccharide (LPS; 5 mg/ml in saline) from E. Coli into the rat molar gingival sulcus. In normal periodontal tissues before LPS application, a small number of MIP-2 and CINC-2 positive cells were seen in junctional epithelium (JE) especially in its coronal half. After topical application of LPS, a prominent increase of MIP-2 and CINC-2 positive JE cells was observed. Almost all JE cells strongly expressed them at day 1 and day 2, and then the number of chemokine-positive cells returned to normal at day 7. Corresponding to these chemokine expressions, LPS application induced a significant increase in number of PMNs in sub-JE area at 1 hour (P <0.01) and 3 hours (P <0.05) and a significant increase in JE area at 1 day(P< 0.05) and 2 days (P <0.01), indicating a dynamic flow of PMNs from the sub-JE area into JE.

These findings indicated that JE cells produced MIP-2 and CINC-2 in response to LPS stimulation and suggested that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation and subsequent periodontal tissue destruction.

Chemokine

Lipopolysaccharide

Polymorphonuclear leukocyte

Periodontitis

Animal study

Medical sciences [ 490 ]

eng

Springer

Copyright (c) 2004 Springer-Verlag. "The original publication is available at www.springerlink.com"

[ISSN] 0948-6143

[DOI] 10.1007/s00418-004-0636-6

[NCID] AA11033302

[DOI] http://dx.doi.org/10.1007/s00418-004-0636-6