Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function
Journal of Pharmaceutical Sciences Volume 96 Issue 1
Page 223-233
published_at 2006
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| File | |
| Title ( eng ) |
Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function
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| Creator |
Patanasethanont Denpong
Nagai Junya
Yumoto Ryoko
Murakami Teruo
Sutthanut Khaetthareeya
Sripanidkulchai Bung-Orn
Yenjai Chavi
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| Source Title |
Journal of Pharmaceutical Sciences
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| Volume | 96 |
| Issue | 1 |
| Start Page | 223 |
| End Page | 233 |
| Abstract |
The purpose of this study was to examine the effects of extracts and flavone derivatives from the rhizome of Kaempferia parviflora on P-glycoprotein (P-gp)-mediated transport in LLC-GA5-COL150, a transfectant cell line of a porcine kidney epithelial cell line LLC-PK1 with human MDR1 cDNA. Ethanol extract obtained from Kaempferia parviflora rhizome significantly increased the accumulation of rhodamine 123 and daunorubicin, P-gp substrates, in LLC-GA5-COL150 cells, but not in LLC-PK1 cells. The aqueous extract also increased the accumulation in LLC-GA5-COL150 cells with lower potency than the ethanol extract. The effects of flavone derivatives isolated from the rhizome of Kaempferia parviflora on P-gp function were examined. Among six flavones tested, 3,5,7,3',4'-pentamethoxyflavone most potently increased the accumulation of rhodamine 123 and daunorubicin in LLC-GA5-COL150 cells in a concentration-dependent manner. In addition, 5,7-dimethoxyflavone to lesser degree increased rhodamine 123 accumulation in LLC-GA5-COL150 cells. In contrast, the other four flavone derivatives had no significant effect on the accumulation of rhodamine 123 in LLC-GA5-COL150 cells in a concentration range tested. These results indicate that extracts and flavone derivatives from the rhizome of Kaempferia parviflora can inhibit P-gp function, which may be useful for overcoming P-gp-mediated multidrug resistance and improving the oral bioavailability of anticancer agents.
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| Keywords |
Multidrug resistance
P-glycoprotein
Cancer chemotherapy
Bioavailability
Cell culture
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| NDC |
Medical sciences [ 490 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
John Wiley & Sons Inc
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| Date of Issued | 2006 |
| Rights |
Copyright (c) 2006 John Wiley & Sons, Inc.
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0022-3549
[DOI] 10.1002/jps.20769
[PMID] 17031860
[NCID] AA00704450
[DOI] http://dx.doi.org/10.1002/jps.20769
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