Regulation of c-myc through intranuclear localization of its RNA subspecies

Shimizu Noriaki

Kawamoto June-ko

Utani Koh-ichi

Biochemical and Biophysical Research Communications

We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be temporally regulated by cell cycle progression. Hybridization with exon- and strand-specific RNA probes indicated that the non-protein coding exon 1 plays a novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA targeting exon 2 localized only with nucleoplasmic foci, where it might interact with the sense strand. Thus, c-myc gene expression may be regulated by intranuclear localization of its RNA.

nucleolus

RNA FISH

nuclear structure and function

post-transcriptional gene regulation

c-myc

Biology [ 460 ]

eng

Academic Press Inc Elsevier Science

Copyright (c) 2007 Elsevier Inc.

[ISSN] 0006-291X

[DOI] 10.1016/j.bbrc.2007.05.201

[NCID] AA00564395

[DOI] http://dx.doi.org/10.1016/j.bbrc.2007.05.201