Intensification of the emission signals using the reflection mirrors mounted to the sample holder of a fluorimeter

Journal of Fluorescence Volume 16 Issue 6 Page 739-742 published_at 2006-11
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Title ( eng )
Intensification of the emission signals using the reflection mirrors mounted to the sample holder of a fluorimeter
Creator
Source Title
Journal of Fluorescence
Volume 16
Issue 6
Start Page 739
End Page 742
Abstract
Effects of the reflection mirrors mounted to the sample holder of a fluorimeter have been investigated. Analytical expressions are given for the emission intensity measured as a function of the concentration or optical density of the sample, in case one or two reflection mirrors are attached beside the sample holder to intensify the emission signals. The emission intensity calculated as a function of the sample concentration agreed well with the experimental data. By mounting two reflection mirrors, the emission signal was intensified by a factor of near 3.5. However, the degree of intensification depended strongly on the sample concentration and the linearity between the sample concentration and the emission intensity deviated significantly with increasing the sample concentration.
Keywords
Signal intensification
Emission
Fluorimetry
Optical density
Reflection mirrors
NDC
Chemistry [ 430 ]
Language
eng
Resource Type journal article
Publisher
Springer
Date of Issued 2006-11
Rights
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com"
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 1053-0509
[DOI] 10.1007/s10895-006-0131-7
[NCID] AA10871550
[DOI] http://dx.doi.org/10.1007/s10895-006-0131-7 isVersionOf