Effect of F-spondin on cementoblastic differentiation of human periodontal ligament cells

Kitagawa Masae

Kudo Yasusei

Iizuka Shinji

Ogawa Ikuko

Abiko Yoshimitsu

Miyauchi Mutsumi

Takata Takashi

Biochemical and Biophysical Research Communications

Cementum is a mineralized tissue produced by cementoblasts covering the roots of teeth that provides for the attachment of periodontal ligament to roots and surrounding alveolar bone. To study the mechanism of proliferation and differentiation of cementoblasts is important for understanding periodontal physiology and pathology including periodontal tissue regeneration. However, the detailed mechanism of the proliferation and differentiation of human cementoblasts is still unclear. We previously established human cementoblast-like (HCEM) cell lines. We thought that comparing the transcriptional profiles of HCEM cells and human periodontal ligament (HPL) cells derived from the same teeth could be a good approach to identify genes that influence the nature of cementoblasts. We identified F-spondin as the gene demonstrating the high fold change expression in HCEM cells. Interestingly, F-spondin highly expressing HPL cells showed similar phenotype of cementoblasts, such as up-regulation of mineralized-related genes. Overall, we identified F-spondin as a promoting factor for cementoblastic differentiation.

F-spondin

Cementoblast

Periodontal ligament

Differentiation

Biology [ 460 ]

eng

Elsevier

Copyright (c) 2006 Elsevier Ltd.

[ISSN] 0006-291X

[DOI] 10.1016/j.bbrc.2006.08.142

[NCID] AA00564395

[PMID] 16965763

[DOI] http://dx.doi.org/10.1016/j.bbrc.2006.08.142 isVersionOf