Involvement of calcineurin-dependent degradation of Yap1p in Ca2+-induced G2 cell-cycle regulation in Saccharomyces cerevisiae

Yokoyama Hiroshi

Mizunuma Masaki

Okamoto Michiyo

Yamamoto Josuke

Hirata Dai

Miyakawa Tokichi

EMBO Reports

The Ca2+-activated pathways in Saccharomyces cerevisiae induce a delay in the onset of mitosis through the activation of Swe1p, a negative regulatory kinase that inhibits the Cdc28p/Clb complex. We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression. YAP1 deletion on a zds1Δ background exacerbated the Ca2+-related phenotype. Yap1p was degraded in a calcineurin-dependent manner when cells were exposed to calcium. In yap1Δ cells, the expression level of the RPN4 gene encoding a transcription factor for the subunits of the ubiquitin - proteasome system was diminished. The deletion of YAP1 gene or RPN4 gene led to the accumulation of Swe1p and Cln2p. Yap1p was a substrate of calcineurin in vivo and in vitro. The calcineurin-mediated Yap1p degradation seems to be a long adaptive response that assures a G2 delay in response to a stress that causes the activation of the calcium signalling pathways.

calcium

calcineurin

Swe1p

Yap1p

yeast

eng

Nature Publishing Group

Copyright (c) 2006 European Molecular Biology Organization

[ISSN] 1469-221X

[DOI] 10.1038/sj.embor.7400647

[NCID] AA11472481

[DOI] http://dx.doi.org/10.1038/sj.embor.7400647 isVersionOf