Although the spread of COVID-19 brought radical change to our lifestyle, its impact on health management of university staff is unknown. We enrolled 1608 Hiroshima University staff aged 40 years or more and compared their health checkup data before and after the spread of COVID-19. After adjustment for age, values of alanine aminotransferase (β= 0.04, P = 0.02), high-density lipoprotein cholesterol (β= -0.07, P = 0.0002), and low-density lipoprotein cholesterol (β= 0.04, P = 0.02) were worsened due to the spread of COVID-19. However, hemoglobin A1c (β= -0.04, P = 0.02) was improved, and values of body mass index, waist circumference, aspartate transaminase, γ-glutamyl transpeptidase, blood glucose, triglyceride, systolic and diastolic blood pressure, and uric acid were unchanged by the spread of COVID-19. For better physical health management of the university staff, individual lifestyle changes due to the spread of COVID-19 need to be considered.