Removal of Gaseous Acetic Acid by Corynebacterium sp. No. 123 Immobilized onto Ceramic Beads

無臭化細菌群の中から、揮発性脂肪酸利用性の強い菌株を30菌株分離した。これらの菌株について2%エタノール中でも酢酸の利用性の強い菌棟を見い出し、これを Comynebacterium 属の細菌であると同定した。本菌株の液体培養を行い、その洗浄菌体を真空下で、セラミックビーズに固定した。無菌下で再培養を行って活性化を行った。これをカラムにつめて、バイオフィルターを作り、3.6×M3kg-1day-1の条件下で約100ppmのガス状酢酸を流すと実験の20日間は除去された。

From the seed culture, thirty bacterial isolates capable of utilizing volatile fatty acids were obtained. An isolate with the highest utilization activity of acetic acid even in the presence of 2% ethanol was selected and identified as Corynebacterium sp. The optimum pH and temperature for the growth were 6~9, and 30~45°C, respectively. This strain was immobilized onto the porous ceramic beads in vacuo, and cultivated for activation at 37° C, for 24 h. The gaseous acetic acid was passed through the beads in a column at a flow rate of 3.6 M3 kg-1day-1. Under these conditions, about one hundred ppm of acetic acid was removed for 20 days of the operation period.