Both acute toxicity tests of hydrogen sulfide and tolerance test to anoxia and anoxia with hydrogen sulfide on Portunus trituberculatus, Metapenaeus monoceros and Macrobrachium nipponense were carried out. The 96hr-LC50 of hydrogen sulfide is 31.5 μgℓ-1 for P. trituberculatus, 35.2μgℓ-1 for M. monoceros, and 51.0μgℓ-1 for M. nipponense. In anoxia, values of median lethal time (LT50) were 1.2 day (28hr), 0.9 day (22hr) and 1.6 day (35hr) for P. trituberculatus, M. monoceros and M. nipponense, respectively. The tolerance of P. trituberculatus and M. monoceros to anoxia is lower than the tolerance of M. nipponense. These results suggest that field populations of P. trituberculatus and M. monoceros are easily affected by natural hypoxia with hydrogen sulfide.