Starch degradation of corn was measured after incubation with ruminal fluids supplemented with six nitrogen sources; urea, casein and corn gluten meal plus four levels of lysine. Ruminal fluids were obtained from sheep fed four diets whose hay to concentrate ratios were 50:50 and 20:80 at 12 or 17% crude protein. Incubations with ruminal fluids from sheep fed the high-protein diets caused higher degradation than those from sheep fed the low-protein diets. The degradation of starch incubated with urea or casein was higher than that with corn gluten meal plus lysine. Additions of lysine to corn gluten meal were not able to enhance starch degradation by any ruminal fluids. Ammonia nitrogen rather than amino acid seems strongly to affect in vitro degradation of starch in corn.