The great geo-disaster occurred in the 2008 Iwate-Miyagi Nairiku Earthquake. In this study, we carried out the microtremor measurement, and calculated microtremor H/V spectrum ratio in 17 seismic stations where the strong ground motion were recorded in the 2008 Iwate-Miyagi Nairiku Earthquake. Furthermore, we computed the horizontal and vertical site amplification factors in the seismic observation stations using the moderate earthquake motion records. The calculated H/V spectra and site amplification factors will contribute to the seismic motion estimation of the damaged areas and clarification of damage mechanisms in the future study.