The calls of artificial autotriploid and autotetraploid males as well as diploid males of Hyla japonica were recorded at 24±0.5℃ in the laboratory and their structure was sonagraphically analyzed. Note duration, note interval and pulse rate were slightly affected with increasing ploidy, while call duration, note rate and spectrum of frequency were not affected. The mean note duration of the calls of diploid, autotriploid and autotetraploid males was 79msec, 64msec, and 59msec, respectively. The mean note intervals of solo and duet calls were 162msec and 205msec, 175msec and 220msec, and 183msec and 231msec in the diploid, autotriploid and autotetraploid males, respectively. The mean pulse rate of the diploid, autotriploid, and autotetraploid calls was 256 pulses/sec, 226 pulses/sec, and 204 pulses/sec, respectively. All the acoustic parameters, including ploidy correlated parameters, varied at a wide range which overlapped to a large extent with those of other ploidies.