Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment

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タイトル ( eng )
Attempts at Cultivation of Mycobacterium leprae in Cell Culture under Regulation of Redox Potential at Environment
タイトル ( jpn )
酸化還元電位を調整した環境におけるらい菌の細胞培養のこころみ
作成者
Matsuo Yoshiyasu
Tatsukawa Hiroshi
収録物名
Hiroshima Journal of Medical Sciences
31
2
開始ページ 141
終了ページ 143
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
Cultivation of Mycobacterium leprae in cell culture was attempted under lower redox potential by adding such reducing agents as reduced glutathione, DL-cysteine hydrochloride, and dithiothreitol to culture medium, and by reduced oxygen tension varying the depth of medium or applying agar suspension technique. During a five years' period since 1976, a slight increase in the number of acid-fast bacilli at harvests was occasionally observed. However, the viability of bacilli recovered was unable to be proven by the mouse foot pad method. It is uncertain whether lower redox potential at environment is strictly requested for cultivation of M. leprae.
著者キーワード
Mycobacterium leprae
Cell culture
Redox potential
内容記述
This work was supported in part by the Japan-US Cooperative Medical Science Program and the Sasakawa Memorial Health Foundation.
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima University School of Medicine
発行日 1982-06-10
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6752102