Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

Makihira Seicho

Shuto Takahiro

Nikawa Hiroki

Okamoto Keishi

Mine Yuichi

Takamoto Yuko

Ohara Masaru

Tsuji Koichiro

International Journal of Molecular Sciences

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.

antimicrobial peptide

titanium surface

MC3T3-E1 cells

Medical sciences [ 490 ]

eng

Molecular Diversity Preservation International-MDPI

Copyright (c) 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

[ISSN] 1422-0067

[DOI] 10.3390/ijms11041458

[NCID] AA12038549

[DOI] http://dx.doi.org/10.3390/ijms11041458