このエントリーをはてなブックマークに追加
ID 48652
本文ファイル
著者
Takahashi, Shiro
Sugiyama, Taiki
Shimomura, Mayuka
Kamada, Yoshihiro
Fujita, Kazutoshi
Nonomura, Norio
Miyoshi, Eiji
Nakano, Miyako 大学院先端物質科学研究科 広大研究者総覧
キーワード
fucosylated haptoglobin
gastroenterological cancer
metastatic prostate cancer
linkage of fucose
site-specific analysis
抄録(英)
Fucosylation is an important type of glycosylation involved in cancer, and fucosylated proteins could be employed as cancer biomarkers. Previously, we reported that fucosylated N-glycans on haptoglobin in the sera of patients with pancreatic cancer were increased by lectin-ELISA and mass spectrometry analyses. However, an increase in fucosylated haptoglobin has been reported observed in various types of cancer. To ascertain if characteristic fucosylation is observed in each cancer type, we undertook site-specific analyses of N-glycans on haptoglobin in the sera of patients with five types of operable gastroenterological cancer (esophageal, gastric, colon, gallbladder, pancreatic), a non-gastroenterological cancer (prostate cancer) and normal controls using ODS column LC-ESI MS. Haptoglobin has four potential glycosylation sites (Asn184, Asn207, Asn211, Asn241). In all cancer samples, monofucosylated N-glycans were significantly increased at all glycosylation sites. Moreover, difucosylated N-glycans were detected at Asn 184, Asn207 and Asn241 in only cancer samples. Remarkable differences in N-glycan structure among cancer types were not observed. We next analyzed N-glycan alditols released from haptoglobin using graphitized carbon column LC-ESI MS to identify the linkage of fucosylation. Lewis-type and core-type fucosylated N-glycans were increased in gastroenterological cancer samples, but only core-type fucosylated N-glycan was relatively increased in prostate cancer samples. In metastatic prostate cancer, Lewis-type fucosylated N-glycan was also increased. These data suggest that the original tissue/cell producing fucosylated haptoglobin is different in each cancer type and linkage of fucosylation might be a clue of primary lesion, thereby enabling a differential diagnosis between gastroenterological cancers and non-gastroenterological cancers.
掲載誌名
Glycoconjugate Journal
33巻
3号
開始ページ
471
終了ページ
482
出版年月日
2016-02-11
出版者
Springer Verlag
ISSN
0282-0080
1573-4986
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
This is a post-peer-review, pre-copyedit version of an article published in Glycoconjugate Journal. The final authenticated version is available online at: https://doi.org/10.1007/s10719-016-9653-7
関連情報URL
部局名
先端物質科学研究科