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ID 48645
本文ファイル
著者
Kuribayashi, Taka-aki
Fujii, Sotaro 大学院生物圏科学研究科 広大研究者総覧
Masanari, Misa
Yamanaka, Masaru
Wakai, Satoshi
Sambongi, Yoshihiro 大学院生物圏科学研究科 広大研究者総覧
キーワード
Deep-sea
Growth environment
5′-Nucleotidase
NaCl tolerance
Shewanella
抄録(英)
Shewanella species are widely distributed in sea, brackish, and fresh water areas, growing psychrophilically or mesophilically, and piezophilically or piezo-sensitively. Here, membrane-bound 5′-nucleotidases (NTases) from deep-sea Shewanella violacea and brackish water Shewanella amazonensis were examined from the aspect of NaCl tolerance in order to gain an insight into protein stability against salt. Both NTases were single polypeptides with molecular masses of ~59 kDa, as determined on mass spectroscopy. They similarly required 10 mM MgCl2 for their activities, and they exhibited the same pH dependency and substrate specificity for 5′-nucleotides. However, S. violacea 5′-nucleotidase (SVNTase) was active enough in the presence of 2.5 M NaCl, whereas S. amazonensis 5′-nucleotidase (SANTase) exhibited significantly reduced activity with the same concentration of the salt. Although SVNTase and SANTase exhibited high sequence identity (69.7%), differences in the ratio of acidic to basic amino acid residues and the number of potential salt bridges maybe being responsible for the difference in the protein stability against salt. 5′-Nucleotidases from these Shewanella species will provide useful information regarding NaCl tolerance, which may be fundamental for understanding bacterial adaptation to growth environments.
内容記述
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan [No. 26240045], a grant from the Japan Society for the Promotion of Science [No. 25-1446], and The Salt Science Research Foundation [No. 1655].
掲載誌名
Extremophiles
21巻
2号
開始ページ
357
終了ページ
368
出版年月日
2017-01-03
出版者
Springer Verlag
ISSN
1431-0651
1433-4909
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
This is a post-peer-review, pre-copyedit version of an article published in Extremophiles. The final authenticated version is available online at: https://doi.org/10.1007/s00792-016-0909-8
関連情報URL
部局名
生物圏科学研究科