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ID 50001
本文ファイル
著者
MYOGA, Hiroshi
キーワード
Phosphatase
Corpus luteum
Cerium
Microslicer
Ultracytochemistry
NDC
医学
抄録(英)
Acid phospatase (AcPase), alkaline phosphatase (AlPase), 5'-nucleotidase (AMPase), Mg-activated adenosine triphospatase (ATPase), thiamine pyrophospatase (TPPase) and glucose-6-phospatase (G6Pase) were investigated in the human corpus luteum of early pregnancy using a cerium-based method in combination with a microslicer.
Ultrastructurally, cerium phosphate reaction product appears as a very fine electron dense precipitate. The AcPase reaction products were exclusively localized in the lysosomes and in two cisternae of the Golgi lamellae on the trans-side. The AlPase and AMPase reaction products were observed in the plasma membrane. The Mg-activated ATPase reaction products were found in the matrix of the mitochondria as well as in the plasma membrane. The TPPase reaction products were present in two cisternae of the Golgi lamellae on the trans-side. The G6Pase reaction products were found in the rough endoplasmic reticulum. This technique can be applied routinely for the demonstration of phosphatase activity because of its reproducibility and specificity when cerium is used as the capturing agent, and yields better results than the lead-based method. In the luteal cells, these several phosphatases might serve to maintain a constant metabolic activity in the early pregnancy.
掲載誌名
Hiroshima Journal of Medical Sciences
36巻
4号
開始ページ
347
終了ページ
355
出版年月日
1987-12
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
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