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ID 14200
本文ファイル
著者
Hong, Chang Soo
Shitashiro, Maiko
Ikeda, Tsukasa
Takiguchi, Noboru
Ohtake, Hisao
Kato, Junichi
キーワード
Aerotaxis
Pseudomonas aeruginosa
Chemotaxis
Behavioral response
Transducer
NDC
生物科学・一般生物学
抄録(英)
It was previously shown that the chemotaxis gene cluster 1 (cheYZABW) was required for chemotaxis. In this study, the involvement of the same cluster in aerotaxis is described and two transducer genes for aerotaxis are identified. Aerotaxis assays of a number of deletion–insertion mutants of Pseudomonas aeruginosa PAO1 revealed that the chemotaxis gene cluster 1 and cheR are required for aerotaxis. Mutant strains which contained deletions in the methyl-accepting chemotaxis protein-like genes tlpC and tlpG showed decreased aerotaxis. A double mutant deficient in tlpC and tlpG was negative for aerotaxis. TlpC has 45 0x1.e0b6p-891mino acid identity with the Escherichia coli aerotactic transducer Aer. The TlpG protein has a predicted C-terminal segment with 89 0dentity to the highly conserved domain of the E. coli serine chemoreceptor Tsr. A hydropathy plot of TlpG indicated that hydrophobic membrane-spanning regions are missing in TlpG. A PAS motif was found in the N-terminal domains of TlpC and TlpG. On this basis, the tlpC and tlpG genes were renamed aer and aer-2, respectively. No significant homology other than the PAS motif was detected in the N-terminal domains between Aer and Aer-2.
掲載誌名
FEMS microbiology letters
231巻
2号
開始ページ
247
終了ページ
252
出版年月日
2004-02
出版者
Elsevier Science B.V.
ISSN
0378-1097
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2004 Federation of European Microbiological Societies.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/S0378-1097(04)00009-6
部局名
先端物質科学研究科