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ID 633
本文ファイル
別タイトル
Electrophoretic Condition for Separation of Myosin Heavy-chain Isoforms
著者
土路 恭子
新畑 茂充
菊地 邦雄
NDC
生物科学・一般生物学
抄録(英)
An electrophoretic separation of a previously undetected myosin heavy-chain (HC) isoform in rodent skeletal muscles, designated as HCIID, could be achieved for the first time by the use of polyacrylamide (PAA) gradient gel of Bar & Pette ((1988) FEBS Lett. 235,153-155). However, when used as a conventional method in our laboratory, their gel system seems not to result in reproducible electrophoretograms. This study was undertaken to examine electrophoretic conditions necessary to identify myosin heavy-chain (HC) isoforms, HCI, HCIIA, HCIIB and HCIID, with special reference to HCIIA and HCIID. In an attempt to explore the effect of the pH in the separating gel on electrophoretogram the gels adjusted to pH8.6 were shown to provide the best and most reproducible results. In the case that PAA gradient gels were adopted on the basis of the original method of Bar & Pette, HCIIA and HCIID isoforms were best distinguished by the gels with 4-11%.0 As characterized by its effect on the mobility of HC isoforms, concentrations of glycerol as well as of PPA were found to be a critical factor. In a series of experiments where PAA gradient gels were used, any gels other than those with 25-30 0lycerol gradient did not have a capacity to separate definitely fast type HC isoforms. Other combinations between concentrations of PAA and glycerol were also examined including homogeneous concentrations. In contrast to the report by Bar & Pette, PAA gradient gels were not essential in distinguishing HCIIA from HCIID. Enhanced electrophoretic mobilities existing between HC isoforms were displayed on homogeneous PAA gels rather than on gradient gels.
掲載誌名
広島大学総合科学部紀要. IV, 理系編
19巻
開始ページ
71
終了ページ
78
出版年月日
1993-12-31
出版者
広島大学総合科学部
寄与者
国立情報学研究所
作成年月日
2006-03-21
ISSN
1340-8364
NCID
SelfDOI
言語
日本語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
総合科学研究科
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