ID 31926
本文ファイル
別タイトル
ウニ形態形成に関わる母系因子の研究
著者
小川 麻里
NDC
動物学
抄録(英)
Expression of genes underlying various developmental processes such as morphogenesis, body plan, etc. is regulated by cascades of transcription factors. Factors or cell fate determinants of maternal origin are supposed to play important roles to drive these cascades. Sea urchin is one of the most useful material to study maternal factors and transcriptional cascades, because of simplicity of its developmental pattern and body plan and of easiness to get synchronized embryos in a large amount. In addition, the simple and quick procedure to introduce exogenous genes into a large number of sea urchin eggs has been developed in our laboratory.

The study in the present thesis has been undertaken to identify some of the maternal factors of sea urchin eggs and to elucidate their role in sea urchin development. In Part 1 of this thesis, detection and identification of maternal factors, poly(A)+ mitochondrial ribosomal type RNA (mt 12S rRNA and mt 16S rRNA), asymmetrically enriched in small micromeres are described. These two mt rRNAs are localize outside of mitochondria and distinguished from intramitochondrial rRNAs. Based on this findings, the author speculates possible role of extramitochondrial mt rRNAs in sea urchin development.

In Part II, the author has detected other maternal factor, HpSox protein, in sea urchin eggs, and found that by binding to a Sox motif in the promoter of the arylsulfatase gene HpSox protein regulates expression of arylsulfatase protein that is related to build up body plan of pluteus larvae.
目次
Contents
General Abstract / p1
General Introduction / p3
 References / p6
Part I Asymmetrical distribution of mitochondrial rRNA into small micromeres of sea urchin embryos / p8
 Abstract / p9
 Introduction / p10
 Materials and Methods / p12
 Results / p17
 Figures / p20
 Discussion / p25
 References / p28
Part II Sox and other regulatory elements in the promoter region of the sea urchin arylsulfatase Gene / p34
 Abstract / p35
 Introduction / p36
 Materials and Methods / p38
 Results / p41
 Figures / p46
 Discussion / p52
 References / p55
言語
英語
NII資源タイプ
学位論文
広大資料タイプ
学位論文
DCMIタイプ
text
フォーマット
application/pdf
権利情報
Copyright(c) by Author
関連情報(references)
Asymmetrical distribution of mitochondrial rRNA into small micromeres of sea urchin embryos. Mari Ogawa, Reiko Amikura, Koji Akasaka, Tsutomu Kinoshita, Satoru Kobayashi and Hiraku Shimada. accepted for Zoological Science
関連情報URL(references)
http://dx.doi.org/10.2108/zsj.16.445
学位記番号
甲第1981号
授与大学
広島大学(Hiroshima University)
学位名
博士(理学)
学位名の英名
Physical Science
学位の種類の英名
doctoral
学位授与年月日
1999-03-25
部局名
理学研究科