ID 31923
本文ファイル
別タイトル
形質転換シロイヌナズナ植物における外来遺伝子近傍のゲノムDNAの解析に関する研究
著者
澤崎 達也
NDC
植物学
目次
CONTENTS
INTRODUCTION / p1
MATERIALS AND METHODS / p4
CHAPTER I STABLE TRANSFORMATION OF ARABIDOPSIS WITH THE BAR GENE USING PARTICLE BOMBARDMENT / p12
 Section1 Comparison of 3 selectable makers using changes in the number of drug-resistant calli on Arabidopsis root tissues / p12
 Section2 Regeneration of bialaphos-resistant Arabidopsis plants / p15
 Section3 Analyses of transgenic Arabidopsis plants / p17
 Section4 Analyses of morphology of transgenic Arabidopsis plants / p22
CHAPTER II CHARACTERIZATION OF JUNCTION REGIONS IN TRANSGENIC ARABIDOPSIS PLANTS OBTAINED BY PARTICLE BOMBARDMENT / p24
 Section1 Cloning of transgene and junction regions in transgenic Arabidopsis / p24
 Section2 Characterization of sequences of junction regions / p27
 Section3 In vitro binding assay of junction regions against tobacco matrices / p30
 Section4 Homology of junction regions / p35
 Section5 Characteristics of sequences of the CT junctions / p36
CHAPTER III INTEGRATION MODEL FOR THE TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT / p39
CONCLUSIONS / p42
ACKNOWLEDGMENTS / p44
REFERENCES / p45
SelfDOI
言語
英語
NII資源タイプ
学位論文
広大資料タイプ
学位論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
ETD
権利情報
Copyright(c) by Author
関連情報(references)
・Sawasaki, T., Seki, M., Anzai, H., Irifune, K. and Morikawa, H. (1994) Stable transformation of Arabidopsis with the bar gene using particle bombardment. Transgenic Res. 3, 279-286.
・Sawasaki, T., Takahashi, M., Goshima, N. and Morikawa, H. Characterization of jucntion regions in transgenic Arabidopsis plants obtained by particle bombaredment. Gene (in press).
関連情報URL(references)
http://dx.doi.org/10.1007/s002990050432
http://dx.doi.org/10.1016/S0378-1119(98)00388-6
学位記番号
甲第1905号
授与大学
広島大学(Hiroshima University)
学位名
博士(理学)
学位名の英名
Science
学位の種類の英名
doctoral
学位授与年月日
1998-09-21
部局名
理学研究科