このエントリーをはてなブックマークに追加
ID 27775
本文ファイル
著者
Nishimichi, Norihisa
Higashikawa, Fumiko
Kinoh, Hiromi H
Tateishi, Yoshiko
Matsuda, Haruo
NDC
医学
抄録(英)
Osteopontin (OPN) is a cytokine and ligand for multiple members of the integrin family. OPN undergoes the in vivo polymerization catalyzed by cross-linking enzyme transglutaminase 2, which consequently increases the bioactivity through enhanced interaction with integrins. The integrin alpha 9 beta 1, highly expressed on neutrophils, binds to the sequence SVVYGLR only after intact OPN is cleaved by thrombin. The SVVYGLR sequence appears to be cryptic in intact OPN because alpha 9 beta 1 does not recognize intact OPN. Because transglutaminase 2-catalyzed polymers change their physical and chemical properties, we hypothesized that the SVVYGLR site might also be exposed on polymeric OPN. As expected, alpha 9 beta 1 turned into a receptor for polymeric OPN, a result obtained by cell adhesion and migration assays with alpha 9-transfected cells and by detection of direct binding of recombinant soluble alpha 9 beta 1 with colorimetry and surface plasmon resonance analysis. Because the N-terminal fragment of thrombincleaved OPN, a ligand for alpha 9 beta 1, has been reported to attract neutrophils, we next examined migration of neutrophils to polymeric OPN using time-lapse microscopy. Polymeric OPN showed potent neutrophil chemotactic activity, which was clearly inhibited by anti-alpha 9 beta 1 antibody. Unexpectedly, mutagenesis studies showed that alpha 9 beta 1 bound to polymeric OPN independently of the SVVYGLR sequence, and further, SVVYGLR sequence of polymeric OPN was cryptic because SVVYGLR-specific antibody did not recognize polymeric OPN. These results demonstrate that polymerization of OPN generates a novel alpha 9 beta 1-binding site and that the interaction of this site with the alpha 9 beta 1 integrin is critical to the neutrophil chemotaxis induced by polymeric OPN.
掲載誌名
Journal of Bioligical Chemistry
284巻
22号
開始ページ
14769
終了ページ
14776
出版年月日
2009
出版者
Amer Soc Biochemistry Molecular Biology Iec
ISSN
0021-9258
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
関連情報URL
部局名
保健管理センター